Our proprietary Seprion ligand for the separation of protein aggregates can be provided coated onto magnetic beads (PAD-Beads) or microplates (PAD-Plates).
(Code: PADB-100-01 for 100 separations, PADB-25-01 for 25 separations)
PAD-Beads are versatile and flexible. They can be used to capture protein aggregates from liquid samples or homogenised tissues. After capture of aggregates and washing of the beads, the aggregates can be eluted and analysed by ELISA or Western blotting for the protein of interest.
PAD-Plates offer an ELISA-like assay. PAD-Plates can be used to capture protein aggregates from liquid samples or tissue homogenates. The Seprion ligand is provided coated onto the microplate wells. After capture of the aggregates in the microplate wells by the ligand and washing of the wells, the immobilised aggregates are detected with an antibody to the protein of interest.
PAD Plates Evaluation Kit
This kit contains 2 microplates each coated with a different flavour of Seprion, SEP1 and SEP2. It also includes two different buffers, CB1 and CB2. This kit is provided for an initial study in order to see which plate and buffer combination works best for the protein of interest. Once the best combination is established the appropriate kit can be ordered (see below). It is probable that our experts may already have experience of the best combination for your protein and can advise.
(Code: PADPCB1 SEP1-01, PADPCB1 SEP2-01, PADPCB2 SEP1-01 or PADPCB2 SEP2-01)
Once the best plate and buffer combination has been identified for your protein, these can be ordered.
PAD-Mab Aggregated IgG EIA test kit
This kit is used for the detection and monitoring of aggregated IgG which can include aggregated monoclonal antibodies, for example humanised monoclonal antibodies for therapeutic application. Aggregated IgG is captured by the Seprion-coated microwells and after washing is detected with an anti-IgG HRP conjugate.
This kit is used for the detection of aggregated beta-amyloid. Aggregated beta-amyloid is captured from the sample by the Seprion-coated microwells and after washing is detected with an anti-beta-amyloid primary antibody and a HRP-labelled secondary antibody.
(Code: RPB100-01 for 100 extractions)
RapiPREP kits can be used to capture bacteria and viruses from a variety of samples. Magnetic beads capture the micro-organisms from the sample matrix. After washing and elution, the micro-organisms can be analysed by culture or PCR. For example RapiPREP kits have been used for the automated processing of swabs for MRSA, Chlamydia, Neisseria etc detection. Please enquire about your particular application and we can tailor the product to your needs.
TB-Beads are designed to capture and concentrate mycobacteria including tuberculosis (TB) from sputum. After capture and washing, the TB can be detected by microscopy, culture or PCR. Please enquire about your particular application and we can tailor the product to your needs.